|Hair Color||Powder blue|
|First Appearance||Banquet of the Golden Witch|
|Portrayed By||Eri Kitamura|
"Nihihihi!"—Chiester 410, Dawn of the Golden Witch
A weapon in a contract with Beatrice.
410 is a kid with a strong personality, whose peculiar style of speech tends to make light of people.
She likes laughing at people who are serious or can never relax, which is why she's so fond of 45 and Lucifer.
Her choice of words may be coarse, but she loves company and gets lonely easily. She gets depressed if there isn't someone talking with her constantly.
410 is particularly skilled when it comes to firing control, and she can wield unparalleled combative force even in close-range fighting.
Like the other Chiester Sisters, Chiester 410's real world counterpart is speculated by Dlanor A. Knox to be one of Kinzo Ushiromiya's Winchester rifles. However, her real world vessel is actually one of four ceramic bunnies owned by Maria Ushiromiya.
Chiester 410 appears as young girl with powder blue hair, bunny ears, and red eyes. She wears the Chiester Sisters Imperial Guard Corps uniform with blue accents. She has the One-Winged Eagle tattooed on her right thigh.
Chiester 410 has a tendency to act snide and frequently looks down upon her opponents.
Banquet of the Golden Witch
Alliance of the Golden Witch
Chiester 410 and Chiester 45 are summoned by Kinzo Ushiromiya to choose the first six sacrifices for the first twilight. The pair kill Natsuhi, Rudolf, Hideyoshi, Genji, Eva, and Rosa. Later, after Shannon and Kanon lead an escape from the dungeon beneath the Kuwadorian, the pair kill Kanon, Shannon, Nanjo, Krauss, and Kyrie as part of the fourth, fifth, fifth, seventh, and eighth twilights.
End of the Golden Witch
Dawn of the Golden Witch
Twilight of the Golden Witch
During the Halloween after-party, Chiester 410 attempts to trick Natsuhi Ushiromiya into drinking tea with her tongue like a dog.
After the Goats swarm Rokkenjima, Chiester 410 helps protect the lower floor of the mansion. Like her sisters, Chiester 410 is rendered incapable of attacking the Goats when they deny her, referring to the Chiester Sisters as "Maria's rabbit dolls."
Chiester 410 appears as a playable character in Ōgon Musōkyoku. She uses long-ranged bow attacks. Her special ability is "Break Boost," which increases guard damage on block. She has story modes with Virgilia, Lucifer, Rosa, EVA-Beatrice, and Lambdadelta.
- Chiester 410 is named after the .410 bore shotgun shell..